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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/10/2018 |
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Data da última atualização: |
04/10/2018 |
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Autoria: |
MACHADO, G. P.; SILVA, R. C.; GUIMARÃES, F. F.; SALINA, A.; LANGONI, H. |
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Afiliação: |
Gustavo P. Machado, Médico Veterinário; Rodrigo C. Silva, Centro de Ciências Agrárias/Universidade Estadual do Norte do Paraná; Felipe F. Guimarães, Departamento de Higiene Veterinária e Saúde Pública/Universidade Estadual Paulista - Unesp; Anelise Salina, Departamento de Higiene Veterinária e Saúde Pública/Universidade Estadual Paulista - Unesp; Hélio Langoni, Departamento de Higiene Veterinária e Saúde Pública/Universidade Estadual Paulista - Unesp. |
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Título: |
Detection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Brazilian mastitic milk goats by multiplex-PCR. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1358-1364, julho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Detecção de Staphylococcus aureus, Streptococcus agalactiae e Escherichia coli em leite caprino mastítico no Brasil, por multiplex-PCR. |
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Conteúdo: |
This study evalueted the prevalence of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in milk samples from 257 goats (513 half-udders) and ten bulk tanks, from ten dairy goat farms of São Paulo State, Brazil, by multiplex-PCR. The samples were screened by microbiological culture (gold-standard), and tested by different multiplex-PCR protocols for the detection of each bacterium. A total of 178 half-udders resulted positive by microbiological culture, with coagulase-negative staphylococci (70%), S. aureus (13.5%), S. intermedius (7.9%), and Enterobacteriaceae (4%) the prevalent pathogens. In other way, multiplex-PCR detected 173 pathogens in 151/523 (28.9%; CI95% 25.2-32.9%) milk samples 144/513 (28.1%) half-udders and 7/10 (70%) bulk tanks, with E. coli (86/162, 51.9%) and S. aureus (50/162, 30.9%) the prevalent ones in half-udders, and S. aureus (6/10, 60%) and E. coli (4/5, 36.4%) in bulk tanks. Multiplex-PCR showed a high performance for the detection of three bacteria at a time in mastitic goat milk direct from half-udders or bulk tanks. Thus, this multiplex-PCR protocol proved to be an adequate tool for the identification of the most common mastitis pathogens, independent of their phenotypic characteristics in the diagnosis of clinical mastitis in goats, allowing a continuous and better vigilance and monitoring the herd, being included in quality programs. |
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Palavras-Chave: |
Cultura microbiológica; Multiplex-PCR; Tanque de expansão. |
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Thesagro: |
Caprino; Escherichia Coli; Leite; Mamite; Staphylococcus Aureus. |
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Thesaurus Nal: |
Goats; Mastitis; Milk; Milk tanks; Streptococcus agalactiae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183918/1/Detection-of-staphylococcus-aureus.pdf
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Marc: |
LEADER 02551naa a2200337 a 4500
001 2096896
005 2018-10-04
008 2018 bl uuuu u00u1 u #d
100 1 $aMACHADO, G. P.
245 $aDetection of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in Brazilian mastitic milk goats by multiplex-PCR.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Detecção de Staphylococcus aureus, Streptococcus agalactiae e Escherichia coli em leite caprino mastítico no Brasil, por multiplex-PCR.
520 $aThis study evalueted the prevalence of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli in milk samples from 257 goats (513 half-udders) and ten bulk tanks, from ten dairy goat farms of São Paulo State, Brazil, by multiplex-PCR. The samples were screened by microbiological culture (gold-standard), and tested by different multiplex-PCR protocols for the detection of each bacterium. A total of 178 half-udders resulted positive by microbiological culture, with coagulase-negative staphylococci (70%), S. aureus (13.5%), S. intermedius (7.9%), and Enterobacteriaceae (4%) the prevalent pathogens. In other way, multiplex-PCR detected 173 pathogens in 151/523 (28.9%; CI95% 25.2-32.9%) milk samples 144/513 (28.1%) half-udders and 7/10 (70%) bulk tanks, with E. coli (86/162, 51.9%) and S. aureus (50/162, 30.9%) the prevalent ones in half-udders, and S. aureus (6/10, 60%) and E. coli (4/5, 36.4%) in bulk tanks. Multiplex-PCR showed a high performance for the detection of three bacteria at a time in mastitic goat milk direct from half-udders or bulk tanks. Thus, this multiplex-PCR protocol proved to be an adequate tool for the identification of the most common mastitis pathogens, independent of their phenotypic characteristics in the diagnosis of clinical mastitis in goats, allowing a continuous and better vigilance and monitoring the herd, being included in quality programs.
650 $aGoats
650 $aMastitis
650 $aMilk
650 $aMilk tanks
650 $aStreptococcus agalactiae
650 $aCaprino
650 $aEscherichia Coli
650 $aLeite
650 $aMamite
650 $aStaphylococcus Aureus
653 $aCultura microbiológica
653 $aMultiplex-PCR
653 $aTanque de expansão
700 1 $aSILVA, R. C.
700 1 $aGUIMARÃES, F. F.
700 1 $aSALINA, A.
700 1 $aLANGONI, H.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 7, p. 1358-1364, julho 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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